It is possible to restrict access level of the Gate users for providing access only through one reader of controller.
Switch over controller to Two-door pass (it is set by connecting straps, see documentation for controller) and configure time zones in such a way as to the first interval referred to the first reader and the second interval referred to the second reader.
For example, to grant access only by the first reader to employee which have the Access level for entry and grant access only by the second reader to employee which have the Access level for exit, make the following settings in the Access Manager module (see Creation of a time zone in the Access Manager software module and Creating access levels sections):